bs-5029R [Primary Antibody]
SAHH Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SAHH

Immunogen Range: 25-125/432


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 191

Swiss Prot: P23526

Source: KLH conjugated synthetic peptide derived from human SAHH

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 48


Cross Reactive Species: Mouse
Rat
Others

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Huang, Xiaohong, Pin Huan, and Baozhong Liu. "A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas." PROTEOMICS (2016).Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain tissue labeled with RABBIT ANTI-SAHH POLYCLONAL ANTIBODY, Unconjugated (bs-5029R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\n


Lane 1: Mouse Kidney lysates; Lane 2: Mouse Skin lysates probed with SAHH Polyclonal Antibody, Unconjugated (bs-5029R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SAHH) Polyclonal Antibody, Unconjugated (bs-5029R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.