bs-5035R [Primary Antibody]
Apolipoprotein A V Polyclonal Antibody
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Host: Rabbit

Target Protein: APOA5

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 116519

Swiss Prot: Q6Q788

Source: KLH conjugated synthetic peptide derived from human APOA5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009].

Size: 100µL

Concentration: 1ug/ul

Applications: IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Predicted Molecular Weight: 39


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

paraformaldehyde-fixed, paraffin embedded mouse liver tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Apolipoprotein A V Polyclonal Antibody, Unconjugated (bs-5035R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining


Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-Apolipoprotein A V Polyclonal Antibody, Unconjugated(bs-5035R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Apolipoprotein A V Polyclonal Antibody, Unconjugated (bs-5035R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.