bs-5097R [Primary Antibody]
NNT Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NNT

Immunogen Range: 981-1086/1086


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23530

Swiss Prot: Q13423

Source: KLH conjugated synthetic peptide derived from human NNT

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane. May play a role in reactive oxygen species (ROS) detoxification in the adrenal gland.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 109


Cross Reactive Species: Human
Mouse
Dog

Predicted Cross Reactive Species: Rat
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Roider, Elisabeth Maria, and David Erich Fisher. "METHODS AND COMPOSITIONS FOR ENHANCING SKIN PIGMENTATION." U.S. Patent No. 20,160,136,070. 19 May 2016.Read more>>
  • Salerno AG et al. Lack of mitochondrial NADP (H)-transhydrogenase expression in macrophages exacerbates atherosclerosis in hypercholesterolemic mice. Biochem J. 2019 Dec 23;476(24):3769-3789. Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-NNT Polyclonal Antibody, Unconjugated (bs-5097R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NNT Polyclonal Antibody, Unconjugated (bs-5097R) at 1:400 overnight at 4°C, DAB staining.