bs-51116R [Primary Antibody]
H3K36me3 Polyclonal Antibody
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DATASHEET

Host: Rabbit

Target Protein: H3K36me3

Clonality: Polyclonal

Isotype: Not Determined

Entrez Gene: 8350

Swiss Prot: P68431

Source: Polyclonal antibody raised in rabbit against histone H3, trimethylated at lysine 36 (H3K36me3), using a KLH-conjugated synthetic peptide

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Size: 50ug

Concentration: Lot Dependent

Applications: WB(1:300-5000)
ELISA(1:500-1000)
dot-blot

Cross Reactive Species: Human
Zebrafish
Drosophila

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Histone extracts (15 μg) from HeLa cells were analysed by Western blot using the H3K36me3 Polyclonal Antibody H3K36me3 (bs-51116R) at 1:1,000 dilution in TBS-Tween containing 5% skimmed milk.


A Dot Blot analysis was performed to test the cross reactivity of H3K36me3 Polyclonal Antibody(bs-51116R) with peptides containing other H3 and H4 modifications and unmodified sequence. 0.2-100 pmol of peptide containing histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The result demonstrates high specificity of the antibody for the modification of interest.