bs-5125R [Primary Antibody]
Frizzled 7 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Frizzled 7

Immunogen Range: 501-574/574


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8324

Swiss Prot: O75084

Source: KLH conjugated synthetic peptide derived from human Frizzled 7

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 60


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Chicken
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Deng, Boya, et al. "MicroRNA-142-3p inhibits cell proliferation and invasion of cervical cancer cells by targeting FZD7." Tumor Biology (2015): 1-9.Read more>>
  • Ye C et al. Overexpression of FZD7 is associated with poor survival in patients with colon cancer. Pathol Res Pract. 2019 May 27:152478.Read more>>
  • Chen Zhong. et al. DL-methionine and DL-methionyl-DL-methionine Increase Intestinal Development and Activate Wnt/-catenin Signaling Activity in Domestic Pigeons (Columba livia). Poultry Sci. 2021 Dec;:101644Read more>>
  • Cheng-long Jin. et al. Lysine Interacts with Frizzled7 to Activate _-Catenin in Satellite Cell-Participated Skeletal Muscle Growth. J Agr Food Chem. 2022;70(12):37453756Read more>>
  • Qin, Ying-chao. et al. L-glutamate requires -catenin signalling through Frizzled7 to stimulate porcine intestinal stem cell expansion. CELL MOL LIFE SCI. 2022 Oct;79(10):1-13Read more>>
  • Anqi Yang. et al. FZD7, Regulated by Non-CpG Methylation, Plays an Important Role in Immature Porcine Sertoli Cell Proliferation. INT J MOL SCI. 2023 Jan;24(7):6179Read more>>
VALIDATION IMAGES

Image provided by the Independent Validation Program (badge number 29805). Lane 1: MDA-MB-231 cell extract, Lane 2: c6/36 mosquito cell extract (non-reactive\nspecies) probed with Rabbit Anti-Frizzled 7 Polyclonal Antibody, Unconjugated (bs-5125R) at 1:100 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:10000 for 60 min at 26˚C. \n


Lane 1: Mouse Muscle cell lysates probed with Frizzled 7 Polyclonal Antibody, Unconjugated (bs-5125R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: HepG2 cell lysates; Lane 2: Mouse Muscle cell lysates; Lane 3: Rat Muscle cell lysates probed with Frizzled 7 Polyclonal Antibody, Unconjugated (bs-5125R) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.