bs-5146R [Primary Antibody]
AKT3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AKT3

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 207

Swiss Prot: P31749

Source: KLH conjugated synthetic peptide derived from human AKT3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(WB=1:500-2000)
ELISA(ELISA=1:5000-10000)

Predicted Molecular Weight: 56


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Sheep
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Huang Xiao-shuang. et al. Mechanism of Action of Acupotomy in Inhibiting Chondrocyte Apoptosis in Rabbits with KOA through the PI3K/Akt Signaling Pathway. Evid-Based Compl Alt. 2020;2020:4241917Read more>>
  • Yu-Mei Jia. et al. Effects on Autophagy of Moxibustion at Governor Vessel Acupoints in APP/PS1double-Transgenic Alzheimers Disease Mice through the lncRNA Six3os1/miR-511-3p/AKT3 Molecular Axis. EVID-BASED COMPL ALT. 2022 Oct 05;2022:3881962Read more>>
  • Qiyu Gan. et al. Prognostic Value and Immune Infiltration of HPV-Related Genes in the Immune Microenvironment of Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma. CANCERS. 2023 Jan;15(5):1419Read more>>
VALIDATION IMAGES

Lane 1: Cerebrum lysates; Lane 2: Testis lysates probed with AKT3 Polyclonal Antibody, Unconjugated (bs-5146R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human SH-SY5Y cell lysates probed with AKT3 Polyclonal Antibody, Unconjugated (bs-5146R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.