bs-5151R-APC [Conjugated Primary Antibody]
Acrosin Polyclonal Antibody, APC Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Acrosin

Immunogen Range: 201-300/421


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 49

Swiss Prot: P10323

Source: KLH conjugated synthetic peptide derived from human Acrosin

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Conjugation: APC

Excitation/ Emission: 650nm/660nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 33/44


Cross Reactive Species: Human
Mouse
Rat
Goat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liang, Xin, et al. "The Noncircadian Function of the Circadian Clock Gene in the Regulation of Male Fertility." Journal of biological rhythms 28.3 (2013): 208-217.Read more>>
  • Deng, Shou‐Long, et al. "Melatonin promotes development of haploid germ cells from early developing spermatogenic cells of Suffolk sheep under in vitro condition." Journal of Pineal Research (2016).Read more>>
  • Deng et al. Melatonin up-regulates the expression of the GATA-4 transcription factor and increases testosterone secretion from Leydig cells through RORα signaling in anin vitrogoat spermatogonial stem cell differentiation culture system. (2017) Oncotarget. 8:110592-110605Read more>>
  • Liu Y et al. Fluoride interferes with sperm fertilizing ability via downregulated SPAM1, ACR and PRSS21 expression in rat epididymis. J Agric Food Chem. 2019 May 8;67(18):5240-5249. Read more>>
  • Yu K et al. In-vitro differentiation of early pig spermatogenic cells to haploid germ cells. Mol Hum Reprod. 2019 Jul 22. pii: gaz043.Read more>>
  • Li Y et al. Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens. Mol Cell Proteomics. 2020 Jun;19(6):1035-1046.Read more>>
  • Ru Yu. et al. ATF6 deficiency damages the development of spermatogenesis in male Atf6 knockout mice. 2021 Dec 13Read more>>
  • Zhang, Guohui. et al. Deficiency of cancer/testis antigen gene CT55 causes male infertility in humans and mice. CELL DEATH DIFFER. 2022 Dec;:1-15Read more>>
VALIDATION IMAGES