bs-5177R [Primary Antibody]
GADD153 (Ser30) Polyclonal Antibody
www.biossusa.com
[email protected]
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DATASHEET

Host: Rabbit

Target Protein: GADD153 Ser30

Modification Site: Ser30

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1649

Source: KLH conjugated synthetic phosphopeptide derived from human GADD153 around the phosphorylation site of Ser30

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 19


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Sheep
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yujie Zhong. et al. Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca 2+ homeostasis. 2021 Mar 02Read more>>
  • Yujie Zhong. et al. Jujuboside A ameliorates high fat diet and streptozotocin induced diabetic nephropathy via suppressing oxidative stress, apoptosis, and enhancing autophagy. Food Chem Toxicol. 2022 Jan;159:112697Read more>>
  • Yujie Zhong. et al. Dioscin relieves diabetic nephropathy via suppressing oxidative stress, apoptosis, and improving mitochondrial quality and quantity control. Food Funct. 2022 FebRead more>>
  • Ali Tugrul Akin. et al. Chloroquine attenuates chronic hypoxia-induced testicular damage via suppressing endoplasmic reticulum stress and apoptosis in experimental rat model. CLIN EXP PHARMACOL P. 2022 May 17Read more>>
  • Yujie Zhong. et al. Diosgenin Ameliorated Type II Diabetes-Associated Nonalcoholic Fatty Liver Disease through Inhibiting De Novo Lipogenesis and Improving Fatty Acid Oxidation and Mitochondrial Function in Rats. NUTRIENTS. 2022 Jan;14(23):4994Read more>>
  • Yujie Zhong. et al. Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy. NUTRIENTS. 2023 Jan;15(9):2164Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-phospho-GADD153 (Ser30) Polyclonal Antibody, Unconjugated (bs-5177R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GADD153 (Ser30) Polyclonal Antibody, Unconjugated (bs-5177R) at 1:400 overnight at 4°C, DAB staining.