bs-5180R
[Primary Antibody]
AXL Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: AXL
Immunogen Range: 151-250/894
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 558
Swiss Prot: P30530
Source: KLH conjugated synthetic peptide derived from human AXL
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TENC1. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 95
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Liu et al. Amlexanox, a selective inhibitor of IKBKE, generates anti-tumoral effects by disrupting the Hippo pathway in human glioblastoma cell lines. (2017) Cell.Death.Dis. 8:e3022Read more>>
- Peng CK et al. Gas6/Axl signaling attenuates alveolar inflammation in ischemia-reperfusion-induced acute lung injury by up-regulating SOCS3-mediated pathway. PLoS One. 2019 Jul 18;14(7):e0219788.Read more>>
- Liang L et al. AXL signaling in primary sensory neurons contributes to chronic compression of dorsal root ganglion-induced neuropathic pain in rats. Mol Pain. 2020 Jan-Dec;16:1744806919900814. Read more>>
- Kong L et al. Qigesan inhibits esophageal cancer cell invasion and migration by inhibiting Gas6/Axl-induced epithelial-mesenchymal transition. Aging (Albany NY). 2020 May 20;12(10):9714-9725.Read more>>
- Siqi Wei. et al. Electro-acupuncture Suppresses AXL Expression in Dorsal Root Ganglion Neurons and Enhances Analgesic Effect of AXL Inhibitor in Spinal Nerve Ligation Induced-Neuropathic Pain Rats. Neurochem Res. 2021 Mar;46(3):504-512Read more>>
- Jianfeng Zhuang. et al. Wogonin Accelerates Hematoma Clearance and Improves Neurological Outcome via the PPAR- Pathway After Intracerebral Hemorrhage. 2020 Sep 12Read more>>
- Zhou, Hang. et al. AXL kinase-mediated astrocytic phagocytosis modulates outcomes of traumatic brain injury. J Neuroinflamm. 2021 Dec;18(1):1-17Read more>>
- Zhang Shengnan. et al. STAT1/SOCS1/3 Are Involved in the Inflammation-Regulating Effect of GAS6/AXL in Periodontal Ligament Cells Induced by Porphyromonas gingivalis Lipopolysaccharide In Vitro. J Immunol Res. 2021;2021:9577695Read more>>
- Mengxue Zhang. et al. Identification and validation of potential novel biomarkers for oral squamous cell carcinoma. Bioengineered. 2021;12(1):8845-8862Read more>>
- Xiaojie Zhang. et al. Bio-engineered Nano-vesicles for IR820 Delivery: A Therapy Platform for Cancer by Surgery and Photothermal Therapy. Nanoscale. 2021 DecRead more>>
- Wangrui Lei. et al. Psoralidin protects against cerebral hypoxia/reoxygenation injury: Role of GAS6/Axl signaling. PHYTOTHER RES. 2022 May 18Read more>>
- Siyi Liu. et al. Nerve injury-induced upregulation of apolipoprotein E in dorsal root ganglion participates in neuropathic pain in male mice. NEUROPHARMACOLOGY. 2023 Feb;224:109372Read more>>
- Zhuang Wan-Ru. et al. Bacterial outer membrane vesicle based versatile nanosystem boosts the efferocytosis blockade triggered tumor-specific immunity. NAT COMMUN. 2023 Mar;14(1):1-15Read more>>
- Yongquan Han. et al. Axl promotes intracranial aneurysm rupture by regulating macrophage polarization toward M1 via STAT1/HIF-1. FRONT IMMUNOL. 2023; 14: 1158758Read more>>
- Weiping Li. et al. Silibinin exerts neuroprotective effects against cerebral hypoxia/reoxygenation injury by activating the GAS6/Axl pathway. TOXICOLOGY. 2023 Aug;:153598Read more>>
- Olivia Justynski. et al. Apoptosis recognition receptors regulate skin tissue repair in mice. ELIFE. 2023 DecRead more>>
- Wangrui Lei. et al. Isopropyl 3-(3,4-dihydroxyphenyl) 2-hydroxypropanoate protects septic myocardial injury via regulating GAS6/Axl-AMPK signaling pathway. BIOCHEM PHARMACOL. 2024 Feb;:116035Read more>>
- Wencheng Di. et al. Pterostilbene protects against H2O2-induced oxidative stress by regulating GAS6/Axl signaling in HL-1 cells. CELL BIOCHEM FUNCT. 2024 Feb;42(2):e3956Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti AXL/UFO Polyclonal Antibody, Unconjugated (bs-5180R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti AXL/UFO Polyclonal Antibody, Unconjugated (bs-5180R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human MKN45 cell lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human FHC cell lysates probed with AXL Polyclonal Antibody, Unconjugated (bs-5180R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Testis lysates; Lane 2:Human Hela cell lysates probed with AXL Polyclonal Antibody, Unconjugated (bs-5180R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.