bs-5194R [Primary Antibody]
AKT1(Thr34) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AKT1 Thr34

Modification Site: Thr34

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 207

Source: KLH conjugated synstic phosphopeptide derived from human AKT1 around the phosphorylation site of Thr34

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 56


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tan et al. ART3 regulates triple-negative breast cancer cell function via activation of Akt and ERK pathways. (2016) Oncotarge. 7:46589-46602Read more>>
  • Li B et al. Resistin up-regulates LPL expression through the PPARγ-dependent PI3K/AKT signaling pathway impacting lipid accumulation in RAW264. 7 macrophages.Cytokine. 2019 Jul;119:168-174.Read more>>
  • Jin Y et al. miR‐496 remedies hypoxia reoxygenation–induced H9c2 cardiomyocyte apoptosis via Hook3‐targeted PI3k/Akt/mTOR signaling pathway activation. J Cell Biochem. 2019 Aug 22. Read more>>
  • Ma Z et al. bFGF promotes neurological recovery from neonatal hypoxicCischemic encephalopathy by IL\1 signaling pathway\mediated axon regeneration. Brain Behav. 2020 Aug;10(8):e01696.Read more>>
  • Shuang Huet al. MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1. Life Sci. 2020 Oct 1;258:118147.Read more>>
  • Han Chen. et al. ERCC6L promotes the progression of hepatocellular carcinoma through activating PI3K/AKT and NF-B signaling pathway. Bmc Cancer. 2020 Dec;20(1):1-1Read more>>
  • Lei Wang. et al. Neuroprotective effect of Lactobacillus plantarum DP189 on MPTP-induced Parkinson's disease model mice. J Funct Foods. 2021 Oct;85:104635Read more>>
  • Zheng Ran. et al. MicrobiomeCmetabolomics analysis reveals the potential effect of verbascoside in alleviating cognitive impairment in db/db mice. FOOD FUNCT. 2023 MaRead more>>
VALIDATION IMAGES

Lane 1: Mouse Embryo lysates; Lane 2: Mouse Lung lysates; Lane 3: A549 lysates; Lane 4: Mouse Cerebrum lysates probed with AKT1(Thr34) Polyclonal Antibody, Unconjugated (bs-5194R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Mouse Heart lysates; Lane 2: Rat Heart lysates probed with phospho-AKT1(Thr34) Polyclonal Antibody, Unconjugated (bs-5194R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Human Siha cell lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Mouse Adrenal gland lysates; Lane 4: Mouse Skeletal muscle lysates; Lane 5: Mouse Ovary lysates; Lane 6: Mouse Lung lysates; Lane 7: Mouse Cerebrum lysates; Lane 8: Mouse Placenta lysates; Lane 9: Human MCF-7 cell lysates; Lane 10: Human A549 cell lysates; Lane 11: Mouse Heart cell lysates probed with AKT1 Polyclonal Antibody, Unconjugated (bs-5194R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.