bs-5198R [Primary Antibody]
ATG16 (Ser287) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ATG16 Ser287

Modification Site: Ser287

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 55054

Swiss Prot: Q676U5

Source: KLH conjugated Synthesised phosphopeptide derived from human ATG16A/ATG16L1 around the phosphorylation site of Ser287

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 68


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

L1 mouse brain lysate probed with Anti phospho-ATG16A(Ser287) Polyclonal Antibody, Unconjugated (bs-5198R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.


Formalin-fixed and paraffin embedded mouse spleen labeled with Anti-phospho-ATG16A(Ser287)Polyclonal Antibody, Unconjugated (bs-5198R) at 1:200 followed by conjugation to the secondary antibody and DAB staining