bs-5277R-Cy5

DATASHEET

Host: Rabbit

Target Protein: CDK2 Thr14

Modification Site: Thr14

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1017

Source: KLH conjugated synthetic phosphopeptide derived from human CDK2 around the phosphorylation site of Thr14 [EG(p-T)YG]

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. cdk2 is a cell cycle protein closely related to Cdc2 (cdk1) that has proved useful as a marker of proliferation. cdk2 binds cyclin type A and E proteins and controls progression into S-phase.

Conjugation: Cy5

Excitation/ Emission: 625,650nm/670nm

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 34

Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.