bs-5293R [Primary Antibody]
phospho-DAXX (Ser671) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: phospho-DAXX (Ser671)

Modification Site: Ser671

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1616

Source: KLH conjugated synthetic phosphopeptide derived from human DAXX around the phosphorylation site of Ser671

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED3 protease family. A novel DD containing molecule was recently cloned from mouse, human and monkey and designated Daxx. Daxx is a death domain containing important intermediate in the Fas mediated apoptosis. Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N terminal kinase (JNK) pathway. It is widely expressed in fetal and adult human and mouse tissue, indicating its important function in Fas signaling pathways.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 81


Cross Reactive Species: Human

Predicted Cross Reactive Species: Cow
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: K562 cell lysates; Lane 2: Hela cell lysates probed with DAXX (Ser671) Polyclonal Antibody, Unconjugated (bs-5293R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human PANC-1 cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HepG2 cell lysates probed with DAXX (Ser671) Polyclonal Antibody, Unconjugated (bs-5293R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human U-2 OS cell lysates; Lane 4: Human MOLT4 cell lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human HeLa cell lysates probed with phospho-DAXX (Ser671) Polyclonal Antibody, Unconjugated (bs-5293R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.


Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-DAXX (Ser671)) Polyclonal Antibody, Unconjugated (bs-5293R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.