bs-53049R [Primary Antibody]
H3K79me1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: H3K79me1

Clonality: Polyclonal

Isotype: Not Determined

Entrez Gene: 8350

Swiss Prot: P68431

Source: Polyclonal antibody raised in rabbit against histone H3 the monomethylated lysine 79 (H3K79me1), using a KLH-conjugated synthetic peptide

Purification: Whole antiserum from rabbit containing 0.05% azide

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Size: 100ul

Concentration: Not Determined

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot was performed on histone extracts from HeLa cells (15 μg) with the H3K79me1 antibody (bs-53049R), diluted 1:1,000 and 1:2000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (Bio-Rad, broad range biotinylated SDS-PAGE standard) is shown on the left, the location of the protein of interest is indicated on the right.


A Dot Blot analysis was performed to test the cross reactivity of the H3K79me1 antibody (bs-53049R) with peptides containing other modifications of histone H3. These include di- and trimethylation of the same lysine and mono-, di- and trimethylation of lysine 9, 27 and 36. One hundred to 0.2 pmol of the peptides were spotted on a membrane. The antibody was used at a dilution of 1:100,000. The figure shows a high specificity of the antibody for the modification of interest.


To determine the titer of the antibody, an ELISA was performed using a serial dilution of the H3K79me1 antibody (bs-53049R) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:30,000.