Host: Rabbit
Target Protein: ASH2
Clonality: Polyclonal
Isotype: Not Determined
Entrez Gene: 9070
Swiss Prot: Q9UBL3
Source: Polyclonal antibody against mouse Ash2, using 3 different KLH-conjugated synthetic peptides, 2 containing an amino acid sequence from the central and 1 containing C-terminal part of the protein
Purification: Whole antiserum from rabbit containing 0.05% azide
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: Not Determined
Applications:
WB(1:500-1000)
ELISA(1:100-500)
IF(1:100-200)
Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
NIH3T3 cells were stained with the Ash2 antibody (bs-53055R) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the Ash2 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two is shown on the right.
To determine the titer, an ELISA was performed using a serial dilution of the Ash2 antibody (bs-53055R). The wells were coated with the peptides used for immunization of the host rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:24,000.