bs-53084R [Primary Antibody]
BMI1 Polyclonal Antibody
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Host: Rabbit

Target Protein: BMI1

Clonality: Polyclonal

Isotype: Not Determined

Entrez Gene: 648

Swiss Prot: P35226

Source: Polyclonal antibody raised in rabbit against mouse BMI1 using two KLH-conjugated synthetic peptides containing a sequence from the central and the C-terminal region of the protein, respectively

Purification: Whole antiserum from rabbit containing 0.05% azide

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Size: 1mg

Concentration: Not Determined

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

To determine the titer of the antibody, an ELISA was performed using a serial dilution of the BMI1 antibody (bs-53084R). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3,100.