bs-53089R [Primary Antibody]
Histone H1.75 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: H1.75

Clonality: Polyclonal

Isotype: Not Determined

Entrez Gene: 3024

Swiss Prot: Q02539

Source: Polyclonal antibody raised in rabbit against histone H1.75, using a KLH-conjugated synthetic peptide

Purification: Whole antiserum from rabbit containing 0.05% azide

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.

Size: 100ul

Concentration: Not Determined

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

To determine the titer of the antibody, an ELISA was performed using a serial dilution of the H1.1 antibody (bs-53089R). The plates were coated with the peptide. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3,000.