bs-53123R [Primary Antibody]
HP1 alpha Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HP1 alpha

Clonality: Polyclonal

Isotype: Not Determined

Entrez Gene: 23468

Swiss Prot: P45973

Source: Polyclonal antibody raised in rabbit against human HP1 alpha (Heterochromatin protein 1 homolog alpha), using the full length recombinant GST tagged protein

Purification: Protein G purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Size: 50ug

Concentration: 2ug/ul

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

HeLa cells were stained with the Bioss antibody against HP1α (Cat. No. bs-53123R) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the HP1α antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.


Western blot was performed on nuclear extracts from HeLa cells (20 μg) with the Bioss antibody against human HP1α (Cat. No. bs-53123R), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the expected location of HP1α, HP1β, and HP1γ is indicated on the right.