bs-5326R-Cy7 [Conjugated Primary Antibody]
FGFR1/FGFR2 (Tyr463/Tyr466) Antibody, Cy7 Conjugated
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Host: Rabbit

Target Protein: FGFR1/FGFR2 Tyr463/Tyr466

Specificity: This phosphorylation site is found at Tyr463 in Human and Mouse FGFR1, and Tyr486 in Rat. It is highly conserved and is homologous to the Tyr466 phosphorylation site in Human and Mouse FGFR2, and the Tyr370 site in Rat FGFR2.

Modification Site: Tyr463/Tyr466

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2260, 2263

Source: KLH conjugated synthetic phosphopeptide derived from human FGFR1 around the phosphorylation site of Tyr463

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through the cellular surface tyrosine kinase receptors. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane region and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, Tyr583, Tyr585, Tyr653, Tyr654, Tyr730 and Tyr766. Tyrosine 653 and 654 are important for catalytic activity of the activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma.

Conjugation: Cy7

Excitation/ Emission: 743nm/767nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 88


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yang Q et al. Anlotinib Suppresses Colorectal Cancer Proliferation and Angiogenesis via Inhibition of AKT/ERK Signaling Cascade. Cancer Manag Res. 2020 Jun 24;12:4937-4948.Read more>>
  • Yang Zhang. et al. Plumbagin Inhibits Proliferation, Migration, and Invasion of Retinal Pigment Epithelial Cells Induced by FGF-2. Tissue Cell. 2021 Oct;72:101547Read more>>
  • Zuyao Chen. et al. Intracellular FGF1 promotes invasion and migration in thyroid carcinoma via HMGA1 independent of FGF receptors. ENDOCR CONNECT. 2023 Mar;-1(aop)Read more>>
VALIDATION IMAGES