DATASHEET
Host:
Rabbit
Target Protein:
Ep300 Ser1834
Modification Site:
Ser1834
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
2033
Source:
KLH conjugated synthetic phosphopeptide derived from human Ep300 around the phosphorylation site of Ser1834
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
KAT3B/p300 is a transcriptional adapter protein (300 kDa)which is characterized by three cysteine and histidine rich regions and its C-terminus specifically binds the adenovirus E1A protein. KAT3B and associated proteins are components of TATA-binding protein (TBP)complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265 kDa nuclear protein designated KAT3A/CBP(for CREB-binding protein). KAT3B and KAT3A are homologous to each other.
PRODUCT SPECIFIC PUBLICATIONS
- Hu Y et al. Ultrasensitive electrochemiluminescence immunosensor for the transcriptional co-activator p300 by using a graphene oxide monolayer and tetrahedral DNA-mediated signal amplification. Mikrochim Acta. 2019 May 2;186(5):325.Read more>>
- Dandan Zheng. et al. Pyruvate Kinase M2 Tetramerization Protects against Hepatic Stellate Cell Activation and Liver?Fibrosis. Am J Pathol. 2020 Nov;190:2267Read more>>
- Merve Ozdemir. et al. HDAC9/p300/F-actin immunoexpression and migration analysis for malignant melanoma stem cell. PATHOL RES PRACT. 2023 Oct;250:154829Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-Ep300 (Ser1834) Polyclonal Antibody, Unconjugated (bs-5339R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cell lysates probed with Ep300 Polyclonal Antibody, Unconjugated (bs-5339R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.