bs-5339R [Primary Antibody]
Ep300 (Ser1834) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Ep300 Ser1834

Modification Site: Ser1834

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2033

Source: KLH conjugated synthetic phosphopeptide derived from human Ep300 around the phosphorylation site of Ser1834

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

KAT3B/p300 is a transcriptional adapter protein (300 kDa)which is characterized by three cysteine and histidine rich regions and its C-terminus specifically binds the adenovirus E1A protein. KAT3B and associated proteins are components of TATA-binding protein (TBP)complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265 kDa nuclear protein designated KAT3A/CBP(for CREB-binding protein). KAT3B and KAT3A are homologous to each other.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 266


Cross Reactive Species: Human
Mouse
Zebrafish

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse
Chicken
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hu Y et al. Ultrasensitive electrochemiluminescence immunosensor for the transcriptional co-activator p300 by using a graphene oxide monolayer and tetrahedral DNA-mediated signal amplification. Mikrochim Acta. 2019 May 2;186(5):325.Read more>>
  • Dandan Zheng. et al. Pyruvate Kinase M2 Tetramerization Protects against Hepatic Stellate Cell Activation and Liver?Fibrosis. Am J Pathol. 2020 Nov;190:2267Read more>>
  • Merve Ozdemir. et al. HDAC9/p300/F-actin immunoexpression and migration analysis for malignant melanoma stem cell. PATHOL RES PRACT. 2023 Oct;250:154829Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-Ep300 (Ser1834) Polyclonal Antibody, Unconjugated (bs-5339R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Hela cell lysates probed with Ep300 Polyclonal Antibody, Unconjugated (bs-5339R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.