bs-5355R [Primary Antibody]
GFAP (Ser8) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GFAP Ser8

Specificity: This phosphorylation site is homologous to that of Ser8 in Rat and Mouse.

Modification Site: Ser8

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2670

Swiss Prot: P14136

Source: KLH conjugated synstic phosphopeptide derived from human GFAP around the phosphorylation site of Ser8

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 48


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Pig

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Mouse Cerebellum lysates; Lane 4: Rat Cerebellum lysates probed with phospho-GFAP (Ser8) Polyclonal Antibody, Unconjugated (bs-5355R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.