bs-5364R [Primary Antibody]
HSF1(Ser307) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HSF1 Ser307

Modification Site: Ser307

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3297

Source: KLH conjugated synthetic phosphopeptide derived from human HSF1 around the phosphorylation site of Ser303

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells.HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 57


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hao, Jie. et al. An IFI6-based hydrogel promotes the healing of radiation-induced skin injury through regulation of the HSF1 activity. J NANOBIOTECHNOL. 2022 Dec;20(1):1-14Read more>>
VALIDATION IMAGES

Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Pancreas tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Lung tissue lysates ; Lane 5: Mouse Large intestine tissue lysates ; Lane 6: Mouse NIH/3T3 cell lysates probed with phospho-HSF1 (Ser307) Polyclonal Antibody, Unconjugated (bs-5364R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.