DATASHEET
Host:
Rabbit
Target Protein:
PLCZ1
Immunogen Range:
351-450/608
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
89869
Swiss Prot:
Q86YW0
Source:
KLH conjugated synthetic peptide derived from human PLCZ1
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.
PRODUCT SPECIFIC PUBLICATIONS
- Meng Wang. et al. A Novel Assisted Oocyte Activation Method Improves Fertilization in Patients With Recurrent Fertilization Failure. Front Cell Dev Biol. 2021; 9: 672081Read more>>
- Xiaopei Zhou. et al. Actl7a deficiency in mice leads to male infertility and fertilization failure. BIOCHEM BIOPH RES CO. 2022 Oct;623:154Read more>>
- Zhou, Xiaopei. et al. A novel homozygous mutation in ACTL7A leads to male infertility. MOL GENET GENOMICS. 2022 Dec;:1-8Read more>>
- Meng Wang. et al. Novel ACTL7A variants in males lead to fertilization failure and male infertility. ANDROLOGY-US. 2023 NovRead more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-PLCZ1 Polyclonal Antibody, Unconjugated(bs-5378R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (Human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLCZ1) Polyclonal Antibody, Unconjugated (bs-5378R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.