bs-5378R [Primary Antibody]
PLCZ1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PLCZ1

Immunogen Range: 351-450/608


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 89869

Swiss Prot: Q86YW0

Source: KLH conjugated synthetic peptide derived from human PLCZ1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 70


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Meng Wang. et al. A Novel Assisted Oocyte Activation Method Improves Fertilization in Patients With Recurrent Fertilization Failure. Front Cell Dev Biol. 2021; 9: 672081Read more>>
  • Xiaopei Zhou. et al. Actl7a deficiency in mice leads to male infertility and fertilization failure. BIOCHEM BIOPH RES CO. 2022 Oct;623:154Read more>>
  • Zhou, Xiaopei. et al. A novel homozygous mutation in ACTL7A leads to male infertility. MOL GENET GENOMICS. 2022 Dec;:1-8Read more>>
  • Meng Wang. et al. Novel ACTL7A variants in males lead to fertilization failure and male infertility. ANDROLOGY-US. 2023 NovRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-PLCZ1 Polyclonal Antibody, Unconjugated(bs-5378R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded (Human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLCZ1) Polyclonal Antibody, Unconjugated (bs-5378R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.