bs-5390R [Primary Antibody]
Seladin 1 Polyclonal Antibody
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: Seladin 1

Immunogen Range: 101-200/516


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1718

Source: KLH conjugated synthetic peptide derived from human Seladin 1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Seladin 1 is highly expressed in brain, adrenal tissues and adrenal tumours, where this enzyme catalyzes the reduction of the delta-24 double bond of sterol intermediates such as desmosterol, during cholesterol biosynthesis. In addition, Seladin 1 has an anti-apoptotic function by reducing caspase 3 activity during oxidative stress-induced apoptosis and protecting cells against amyloid-beta peptide-induced apoptosis. Several studies have identified a reduction in expression levels of this enzyme in brain regions affected by Alzheimer's disease (hippocampus and the subventricular zone).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 55


Cross Reactive Species: Mouse
Rat
Pig
Others

Predicted Cross Reactive Species: Human
Dog
Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang XD et al. MiR-26a promotes apoptosis of porcine granulosa cells by targeting the 3β-hydroxysteroid-Δ24-reductase gene. Asian-Australas J Anim Sci. 2019,July 1.Read more>>
VALIDATION IMAGES

Antigen: bs-5390P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \nTMB staining; Read the data in Microplate Reader by 450nm\n


Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Seladin 1 Polyclonal Antibody, Unconjugated (bs-5390R) at 1:200 overnight at 4°C, DAB staining.