bs-5392R [Primary Antibody]
HOMER3 (Thr36) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HOMER3 Thr36

Modification Site: Thr36

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9454

Swiss Prot: Q9NSC5

Source: KLH conjugated synthetic phosphopeptide derived from human HOMER3 around the phosphorylation site of Thr36 [AL(p-T)VS]

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 40


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

A549 lysate probed with Rabbit Anti-HOMER3 (Thr36) Polyclonal Antibody (bs-5392R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.


U937 cell lysates probed with HOMER3 Polyclonal Antibody, Unconjugated (bs-5392R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HOMER3 (Thr36) Polyclonal Antibody, Unconjugated (bs-5392R) at 1:400 overnight at 4°C, DAB staining.