bs-5449R [Primary Antibody]
IGF1R (Tyr1165 + Tyr1166) Antibody
www.biossusa.com
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Host: Rabbit

Target Protein: IGF1R Tyr1165 + Tyr1166

Modification Site: Tyr1165 + Tyr1166

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3480

Swiss Prot: P08069

Source: KLH conjugated synthetic phosphopeptide derived from human IGF1R around the phosphorylation site of Tyr1165+Tyr1166

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)

Predicted Molecular Weight: 150


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse Kidney cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF1R (Tyr1165 + Tyr1166) Antibody（bs-5449R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).