bs-5452R [Primary Antibody]
ITGB3(Tyr785) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ITGB3 Tyr785

Modification Site: Tyr785

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3690

Source: KLH conjugated synthetic phosphopeptide derived from human ITGB3 around the phosphorylation site of Tyr785

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The ITGB3 (Integrin beta chain beta 3) protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 87


Predicted Cross Reactive Species: Human
Mouse
Rat
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Lane 1: Human HUVEC cell Lysates; Lane 2: Human U-87 MG cell Lysates; Lane 3: Human Hela cell Lysates; Lane 4: Human Jurkat cell Lysates. Probed with phospho-ITGB3 polyclonal Antibody, unconjugated (bs-5452R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human U87MG cell lysates; Lane 2: Human DU145 cell lysates probed with phospho-ITGB3 (Tyr785) Polyclonal Antibody, Unconjugated (bs-5452R) at 1:200 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.