Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IFNAR1(Ser535+Ser538) Polyclonal Antibody, Unconjugated (bs-5457R) at 1:400 overnight at 4°C, DAB staining.


U-937 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IFNAR1(Ser535+Ser538) Antibody（bs-5457R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).


K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IFNAR1(Ser535+Ser538) Antibody(bs-5457R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IFNAR1(Ser535+Ser538)) Polyclonal Antibody, Unconjugated (bs-5457R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.