bs-5476R [Primary Antibody]
P38 MAPK (Thr180) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: P38 MAPK Thr180

Modification Site: Thr180

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1432

Source: KLH conjugated synthetic phosphopeptide derived from human MAPK14 around the phosphorylation site of Thr180

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

p38 is a 38 kDa Stress Activated Protein Kinase/Map Kinase (SAPK/MAPK) that is fully activated by dual phosphorylation on threonine 180 and tyrosine 182, within the activation loop. p38 MAPK plays a critical role in the initiation of G2 delay after ultraviolet radiation; gene knock out studies have also revealed a critical role for p38 in cardiac remodeling. Downstream targets of p38 include the transcription factors ELK1 and ATF2 and the kinases MAPKAPK2 and MAPKAPK5. p38 MAPK plays a role in the production of IL6 and is thought to stabilize erythropoietin production during hypoxic stress. It is activated by environmental stress, many proinflammatory cytokines and lipopolysaccharide. Dual phosphorylation by MAP2K3 and MAP2K6 is required for activation of p38 MAPK. It interacts with MAX, Cdc25B, Cdc25C and binds to the kinase interaction domain in the protein tyrosine phosphatase PTPRR; this interaction retains p38 MAPK in the cytoplasm.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 41


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gong et al. Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling. (2018) Med.Sci.Monit. 24:3514-3523Read more>>
  • Jian Wang. et al. Lactobacillus salivarius ameliorated Mycoplasma gallisepticum-induced inflammatory injury and secondary Escherichia coli infection in chickens: Involvement of intestinal microbiota. Vet Immunol Immunop. 2021 Mar;233:110192Read more>>
  • Le Zhuang. et al. Evaluation of the effects of IL?22 on the proliferation and differentiation of keratinocytes in?vitro. Mol Med Rep. 2020 Oct;22(4):2715-2722Read more>>
  • Jian Wang. et al. A respiratory commensal bacterium acts as a risk factor for Mycoplasma gallisepticum infection in chickens. Vet Immunol Immunop. 2020 Dec;230:110127Read more>>
  • Yang Zhang. et al. Plumbagin Inhibits Proliferation, Migration, and Invasion of Retinal Pigment Epithelial Cells Induced by FGF-2. Tissue Cell. 2021 Oct;72:101547Read more>>
  • Tianqing Huang. et al. Screening and Validation of p38 MAPK Involved in Ovarian Development of Brachymystax lenok. Front Vet Sci. 2022; 9: 752521Read more>>
  • Junling Zhao. et al. The Ameliorative Effect of JNK Inhibitor D-JNKI-1 on Neomycin-Induced Apoptosis in HEI-OC1 Cells. Front Mol Neurosci. 2022; 15: 824762Read more>>
  • Gong Liang. et al. Protective Effects of Vitamin C against Neomycin-Induced Apoptosis in HEI-OC1 Auditory Cell. NEURAL PLAST. 2022;2022:1298692Read more>>
  • Tianqing Huang. et al. miR-301b-5p and its target gene nfatc2ip regulate inflammatory responses in the liver of rainbow trout (Oncorhynchus mykiss) under high temperature stress. ECOTOX ENVIRON SAFE. 2022 Sep;242:113915Read more>>
  • Zhao, Lei. et al. Lactobacillus plantarum S9 alleviates lipid profile, insulin resistance, and inflammation in high-fat diet-induced metabolic syndrome rats. SCI REP-UK. 2022 Sep;12(1):1-1Read more>>
  • Dan-Ping Xie. et al. Peroxiredoxin 5 protects HepG2 cells from ethyl -carboline-3-carboxylate-induced cell death via ROS-dependent MAPK signalling pathways. J CANCER. 2022 Sep 6;13(11):3258-3267Read more>>
  • Yang Wang. et al. Role of GADD45A in myocardial ischemia/reperfusion through mediation of the JNK/p38 MAPK and STAT3/VEGF pathways. INT J MOL MED. 2022 Dec;50(6):1-11Read more>>
  • Muzhe Li. et al. STS load PCL- MECM based hydrogel hybrid scaffold promote meniscal regeneration via modulating macrophage phenotype polarization. BIOMATER SCI-UK. 2023 JaRead more>>
  • Yifan Zhu. et al. Discovery of Selective P2Y6R Antagonists with High Affinity and In Vivo Efficacy for Inflammatory Disease Therapy. J MED CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Lihui Ni. et al. The combination of insulin and linezolid ameliorates Staphylococcus aureus pneumonia in individuals with diabetes via the TLR2/MAPKs/NLRP3 pathway. INT J BIOL MACROMOL. 2023 Jul;242:12475Read more>>
  • Ruyi Qu. et al. Glucocorticoids improve the balance of M1/M2 macrophage polarization in experimental autoimmune uveitis through the P38MAPK-MEF2C axis. INT IMMUNOPHARMACOL. 2023 Jul;120:110392Read more>>
  • Huawei Liu. et al. Integrated multi-omics reveals the beneficial role of chlorogenic acid in improving the growth performance and immune function of immunologically-stressed broilers. ANIM NUTR. 2023 MayRead more>>
  • Kan Shunli. et al. Resveratrol improves the prognosis of rats after spinal cord injury by inhibiting mitogen-activated protein kinases signaling pathway. SCI REP-UK. 2023 Nov;13(1):1-11Read more>>
  • Xinyun Qin. et al. Regulation of the intestinal flora using polysaccharides from Callicarpa nudiflora Hook to alleviate ulcerative colitis and the molecular mechanisms involved. INT J BIOL MACROMOL. 2024 Feb;258:128887Read more>>