bs-5483R [Primary Antibody]
MEF2C(Thr300) Polyclonal Antibody
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Host: Rabbit

Target Protein: MEF2C Thr300

Modification Site: Thr300

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4208

Source: KLH conjugated synthetic phosphopeptide derived from human MEF2C around the phosphorylation site of Thr300

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

MEF2C is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. This protein controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It may also be involved in neurogenesis and in the development of cortical architecture.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 51


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Cow
Pig
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Muscle lysates; Lane 2: Mouse Lymph node lysates probed with MEF2C(Thr300) Polyclonal Antibody, Unconjugated (bs-5483R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Muscle tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Muscle tissue lysates; Lane 5: Rat Spleen tissue lysates probed with phospho-MEF2C (Thr300) Polyclonal Antibody, Unconjugated (bs-5483R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.