bs-5486R [Primary Antibody]
ERK5 (Ser731+Thr733) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ERK5 Ser731+Thr733

Specificity: Recognizes Ser721+Thr723 in mice and rats

Modification Site: Ser731+Thr733

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5598

Swiss Prot: Q13164

Source: KLH conjugated synthetic phosphopeptide derived from human ERK5 around the phosphorylation sites of Ser731+Thr733

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 90


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Muzhe Li. et al. STS load PCL- MECM based hydrogel hybrid scaffold promote meniscal regeneration via modulating macrophage phenotype polarization. BIOMATER SCI-UK. 2023 JaRead more>>
  • Yang Ran. et al. Over-expression of miR-3584-5p Represses Nav1.8 Channel Aggravating Neuropathic Pain caused by Chronic Constriction Injury. MOL NEUROBIOL. 2023 Jun;:1-19Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin-embedded human lung cancer labeled with Rabbit Anti-ERK5 (Ser731+Thr733) Polyclonal Antibody, Unconjugated(bs-5486R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.


Formalin-fixed and paraffin embedded human myometrium labeled with Anti phospho-ERK5(Ser731+Thr733)Polyclonal Antibody, Unconjugated (bs-5486R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK5 ( Ser731+Thr733) Polyclonal Antibody, Unconjugated (bs-5486R) at 1:400 overnight at 4°C, DAB staining.


Lane 1: Mouse Muscle tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Rat Muscle tissue lysates; Lane 5: Rat Cerebrum tissue lysates probed with phospho-ERK5 ( Ser731+Thr733) Polyclonal Antibody, Unconjugated (bs-5486R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-ERK5 ( Ser731+Thr733)) Polyclonal Antibody, Unconjugated (bs-5486R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-ERK5 ( Ser731+Thr733)) Polyclonal Antibody, Unconjugated (bs-5486R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.