bs-55002R [Primary Antibody]
ADRBK1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ADRBK1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 156

Swiss Prot: P25098

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 440-689 of human ADRBK1 (NP_001610.2).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 79kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ADRBK1 Polyclonal Antibody, Unconjugated (bs-55002R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human esophageal carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ADRBK1 Polyclonal Antibody, Unconjugated (bs-55002R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ADRBK1 Polyclonal Antibody, Unconjugated (bs-55002R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: 293T cell lysates; Lane 2: ADRBK1 knockout (KO) 293T cell lysates probed with ADRBK1 Polyclonal Antibody, Unconjugated (bs-55002R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: HT-29 cell lysates; Lane 2: Mouse Spleen lysates; Lane 3: Mouse Brain lysates probed with ADRBK1 Polyclonal Antibody, Unconjugated (bs-55002R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.