Host: Rabbit
Target Protein: BAZ1B
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9031
Swiss Prot: Q9UIG0
Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BAZ1B (NP_115784.1).
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 170kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BAZ1B Polyclonal Antibody, Unconjugated (bs-55022R) at 1:100 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BAZ1B Polyclonal Antibody, Unconjugated (bs-55022R) at 1:100 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BAZ1B Polyclonal Antibody, Unconjugated (bs-55022R) at 1:100 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BAZ1B Polyclonal Antibody, Unconjugated (bs-55022R) at 1:100 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse heart ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BAZ1B Polyclonal Antibody, Unconjugated (bs-55022R) at 1:100 overnight at 4°C, DAB staining.
Lane 1: SW480 cell lysates; Lane 2: BT-474 cell lysates; Lane 3: SKOV3 cell lysates; Lane 4: Hela cell lysates; Lane 5: HepG2 cell lysates; Lane 6: Mouse thymus lysates probed with BAZ1B Polyclonal Antibody, Unconjugated (bs-55022R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: BAZ1B knockout (KO) HeLa cell lysates probed with BAZ1B Polyclonal Antibody, Unconjugated (bs-55022R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (BAZ1B) polyclonal Antibody, Unconjugated (bs-55022R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.