bs-55025R [Primary Antibody]
beta 2 Microglobulin Polyclonal Antibody
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: beta 2 Microglobulin

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 567

Swiss Prot: P61769

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human beta 2 Microglobulin (NP_004039.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
IP(1-2ug)
IHC

Predicted Molecular Weight: 13kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with beta 2 Microglobulin Polyclonal Antibody, Unconjugated (bs-55025R) at 1:100 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with beta 2 Microglobulin Polyclonal Antibody, Unconjugated (bs-55025R) at 1:100 overnight at 4°C, DAB staining.


Lane 1: 293T cell lysates; Lane 2: beta 2 Microglobulin knockout (KO) 293T cell lysates probed with beta 2 Microglobulin Polyclonal Antibody, Unconjugated (bs-55025R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Jurkat cell lysates; Lane 2: COS7 cell lysates; Lane 3: Hela cell lysates; Lane 4: K562 cell lysates; Lane 5: Mouse Liver lysates; Lane 6: Mouse Spleen lysates probed with beta 2 Microglobulin Polyclonal Antibody, Unconjugated (bs-55025R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.