bs-55032R-Cy5.5

DATASHEET

Host: Rabbit

Target Protein: Caspase-3

Immunogen Range: 55-160/277

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 836

Swiss Prot: P42574

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase-3 (NP_004337.2).

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Conjugation: Cy5.5

Excitation/ Emission: 675nm/694nm

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:500-2000)
IF(1:100-500)

Predicted Molecular Weight: 31

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.