bs-55039R [Primary Antibody]
CD44 Polyclonal Antibody
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD44

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 960

Swiss Prot: P16070

Source: Recombinant human CD44

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µL

Concentration: 1mg/ml

Applications: WB(1:500-2000)

Predicted Molecular Weight: 82


Cross Reactive Species: Human
Rabbit

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yu He. et al. Single-Cell RNA Sequencing Unravels Distinct Tumor Microenvironment of Different Components of Lung Adenocarcinoma Featured as Mixed Ground-Glass Opacity. FRONT IMMUNOL. 2022; 13: 903513Read more>>
  • Juan Cen. et al. A Water-Soluble Quercetin Conjugate with Triple Targeting Exerts Neuron-Protective Effect on Cerebral Ischemia by Mitophagy Activation. ADV HEALTHC MATER. 2022 Sep;:2200817Read more>>
  • Ma Caiyun. et al. Characteristics and Multilineage Differentiation of a Novel Type of Pulmonary Mesenchymal Stem Cells Derived from Goose Embryos. BRAZ ARCH BIOL TECHN. 2024 Apr;67:e24230139Read more>>
VALIDATION IMAGES

Lane 1: Hela cell lysates; Lane 2: CD44 knockout (KO) HeLa cell lysates probed with CD44 Polyclonal Antibody, Unconjugated (bs-55039R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse spleen cell lysates probed with CD44 Polyclonal Antibody, Unconjugated (bs-55039R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human A549 cell lysates; Lane 2: Human U2os cell lysates; Lane 3: Human MDA-MB-231 cell lysates; Lane 4: Human Hela cell lysates probed with CD44 Polyclonal Antibody, Unconjugated (bs-55039R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human Hela cell (Control) lysates; Lane 2: CD44 knockout (KO) Hela cell lysates probed with CD44 Polyclonal Antibody, Unconjugated (bs-55039R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human Hela cell lysates; Lane 2: Human Panc-1 cell lysates; Lane 3: Human Huvec cell lysates; Lane 4: Human A549 cell lysates probed with CD44 Polyclonal Antibody, Unconjugated (bs-55039R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.