bs-55045R [Primary Antibody]
COMT Polyclonal Antibody
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Host: Rabbit

Target Protein: COMT

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1312

Swiss Prot: P21964

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 42-221 of human COMT (NP_009294.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC(1:50-200)
IF

Predicted Molecular Weight: 24kDa/30kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: 293T cell lysates; Lane 2: COMT knockout (KO) 293T cell lysates probed with COMT Polyclonal Antibody, Unconjugated (bs-55045R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.