bs-55046R [Primary Antibody]
CRKL Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CRKL

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1399

Swiss Prot: P46109

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CRKL (NP_005198.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 33kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cell lysates; Lane 2: CRKL knockout (KO) HeLa cell lysates probed with CRKL Polyclonal Antibody, Unconjugated (bs-55046R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: LO2 cell lysates; Lane 2: Jurkat cell lysates; Lane 3: SKOV3 cell lysates; Lane 4: Mouse Brain lysates; Lane 5: Rat heart cell lysates probed with CRKL Polyclonal Antibody, Unconjugated (bs-55046R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.