DATASHEET
Host:
Rabbit
Target Protein:
CRKL
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
1399
Swiss Prot:
P46109
Source:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CRKL (NP_005198.1).
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
VALIDATION IMAGES
Lane 1: Hela cell lysates; Lane 2: CRKL knockout (KO) HeLa cell lysates probed with CRKL Polyclonal Antibody, Unconjugated (bs-55046R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: LO2 cell lysates; Lane 2: Jurkat cell lysates; Lane 3: SKOV3 cell lysates; Lane 4: Mouse Brain lysates; Lane 5: Rat heart cell lysates probed with CRKL Polyclonal Antibody, Unconjugated (bs-55046R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.