bs-55049R [Primary Antibody]
CSPG4 Polyclonal Antibody
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Host: Rabbit

Target Protein: CSPG4

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1464

Swiss Prot: Q6UVK1

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human CSPG4 (NP_001888.2).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(1:50-200)

Predicted Molecular Weight: 250kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cell lysates; Lane 2: CSPG4 knockout (KO) HeLa cell lysates probed with CSPG4 Polyclonal Antibody, Unconjugated (bs-55049R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: A375 cell lysates; Lane 2: Hela cell lysates probed with CSPG4 Polyclonal Antibody, Unconjugated (bs-55049R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.