VALIDATION IMAGES
Lane 1: Human Jurkat cell lysates; Lane 2: Human H-460 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Mouse Brain lysates; Lane 5: Rat Brain lysates probed with DFFA Polyclonal Antibody, Unconjugated (bs-55054R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: DFFA knockout (KO) HeLa cell lysates probed with DFFA Polyclonal Antibody, Unconjugated (bs-55054R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (DFFA ) polyclonal Antibody, Unconjugated (bs-55054R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuc
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (DFFA ) polyclonal Antibody, Unconjugated (bs-55054R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuc