bs-55060R [Primary Antibody]
EEF2K Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: EEF2K

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 29904

Swiss Prot: O00418

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 82kDa


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cell lysates; Lane 2: EEF2K knockout (KO) HeLa cell lysates probed with EEF2K Polyclonal Antibody, Unconjugated (bs-55060R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: HepG2 cell lysates; Lane 2: Hela cell lysates; Lane 3: Lovo cell lysates; Lane 4: 22Rv1 cell lysates; Lane 5: 293T cell lysates; Lane 6: Mouse Spleen lysates probed with EEF2K Polyclonal Antibody, Unconjugated (bs-55060R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (EEF2K) polyclonal Antibody, Unconjugated (bs-55060R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.