bs-55065R [Primary Antibody]
EIF4EBP1 Polyclonal Antibody
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Host: Rabbit

Target Protein: EIF4EBP1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1978

Swiss Prot: Q13541

Source: A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human EIF4EBP1 (NP_004086.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(ICC)(1:50-200)
IHC(1:50-200)
IF

Predicted Molecular Weight: 12kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human MCF-7 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Mouse NIH/3T3 cell lysates; Lane 5: Mouse fat lysates; Lane 6: Mouse heart lysates probed with EIF4EBP1 Polyclonal Antibody, Unconjugated (bs-55065R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: EIF4EBP1 knockout (KO) HeLa cell lysates probed with EIF4EBP1 Polyclonal Antibody, Unconjugated (bs-55065R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (EIF4EBP1) polyclonal Antibody, Unconjugated (bs-55065R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.