bs-55091R [Primary Antibody]
GSS Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GSS

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2937

Swiss Prot: P48637

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human GSS (NP_000169.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 40kDa/52kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: DU145 cell lysates; Lane 2: K562 cell lysates; Lane 3: 293T cell lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Spleen lysates; Lane 6: Mouse Brain lysates; Lane 7: Rat Kidney lysates probed with GSS Polyclonal Antibody, Unconjugated (bs-55091R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: 293T cell lysates; Lane 2: GSS knockout (KO) 293T cell lysates probed with GSS Polyclonal Antibody, Unconjugated (bs-55091R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.