bs-55094R [Primary Antibody]
HDAC8 Polyclonal Antibody
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Host: Rabbit

Target Protein: HDAC8

Immunogen Range: 1-377/377


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 55869

Swiss Prot: Q9BY41

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human HDAC8 (NP_060956.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC(1:50-200)
IF(1:50-200)

Predicted Molecular Weight: 15kDa/16kDa/17kDa/28kDa/29kDa/31kDa/41kDa


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC8 Polyclonal Antibody, Unconjugated (bs-55094R) at 1:100 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC8 Polyclonal Antibody, Unconjugated (bs-55094R) at 1:100 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC8 Polyclonal Antibody, Unconjugated (bs-55094R) at 1:100 overnight at 4°C, DAB staining.


Lane 1: 293T cell lysates; Lane 2: Mouse Lung lysates probed with HDAC8 Polyclonal Antibody, Unconjugated (bs-55094R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: HDAC8 knockout (KO) HeLa cell lysates probed with HDAC8 Polyclonal Antibody, Unconjugated (bs-55094R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates probed with HDAC8 Polyclonal Antibody, Unconjugated (bs-55094R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.