bs-55098R [Primary Antibody]
HMGB1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HMGB1

Specificity: KO-Validated

Immunogen Range: 100-200/215


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3146

Swiss Prot: P09429

Source: KLH conjugated synthetic peptide derived from human HMGB1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 25


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMGB1 Polyclonal Antibody, Unconjugated (bs-55098R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMGB1 Polyclonal Antibody, Unconjugated (bs-55098R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMGB1 Polyclonal Antibody, Unconjugated (bs-55098R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Mouse Lung tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Rat Cerebrum tissue lysates ; Lane 5: Human Jurkat cell lysates ; Lane 6: Human Hela cell lysates ; Lane 7: Human HepG2 cell lysates ; Lane 8: Human Molt-4 cell lysates probed with HMGB1 Polyclonal Antibody, Unconjugated (bs-55098R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.