bs-55102R [Primary Antibody]
HPRT1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HPRT1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3251

Swiss Prot: P00492

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human HPRT1 (NP_000185.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 24kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: 293T cell lysates; Lane 2: HPRT1 knockout (KO) 293T cell lysates probed with HPRT1 Polyclonal Antibody, Unconjugated (bs-55102R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: MCF-7 lysates; Lane 2: HL-60 cell lysates; Lane 3: SW480 cell lysates; Lane 4: 293T cell lysates; Lane 5: Hela cell lysates; Lane 6: Mouse brain lysates; Lane 7: Mouse Liver lysates probed with HPRT1 Polyclonal Antibody, Unconjugated (bs-55102R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HPRT1 ) polyclonal Antibody, Unconjugated (bs-55102R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.