bs-55111R [Primary Antibody]
IL18 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL18

Immunogen Range: 37-193/193


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3606

Swiss Prot: Q14116

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 37-193 of human IL18 (NP_001553.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 21


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yunhai Luo. et al. SET8 mitigates hepatic ischemia/reperfusion injury in mice by suppressing MARK4/NLRP3 inflammasome pathway. Life Sci. 2021 May;273:119286Read more>>
  • Run-Feng Miao. et al. LncRNA CDKN2B-AS1 interacts with LIN28B to exacerbate sepsis-induced acute lung injury by inducing HIF-1/NLRP3-mediated pyroptosis. KAOHSIUNG J MED SCI. 2023 JuRead more>>
  • Wenliang Zha. et al. Mitochonic acid 5 rescues cardiomyocytes from doxorubicin-induced toxicity via repressing the TNF-/NF-B/NLRP3-mediated pyroptosis. INT IMMUNOPHARMACOL. 2023 Oct;123:110736Read more>>
VALIDATION IMAGES

Lane 1: Hela cell lysates; Lane 2: IL18 knockout (KO) HeLa cell lysates probed with IL18 Polyclonal Antibody, Unconjugated (bs-55111R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human Hela cell lysates; Lane 2: Mouse lung lysates probed with IL18 Polyclonal Antibody, Unconjugated (bs-55111R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Tissue/cell: L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (IL18 ) polyclonal Antibody, Unconjugated (bs-55111R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Tissue/cell: U2-OScell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (IL18 ) polyclonal Antibody, Unconjugated (bs-55111R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.