bs-55128R [Primary Antibody]
MAP2K4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MAP2K4

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6416

Swiss Prot: P45985

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 300-399 of human MAP2K4 (NP_003001.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 44kDa/45kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cell lysates; Lane 2: MAP2K4 knockout (KO) HeLa cell lysates probed with MAP2K4 Polyclonal Antibody, Unconjugated (bs-55128R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: SH-SY5Y cell lysates; Lane 2: BT-474 cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: Mouse Thymus lysates; Lane 5: Mouse Liver lysates; Lane 6: Rat Liver lysates probed with SIRT4 Polyclonal/ Monoclonal Antibody, Unconjugated (bs-55128R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.