bs-55131R [Primary Antibody]
MLKL Polyclonal Antibody
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Host: Rabbit

Target Protein: MLKL

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 74568

Swiss Prot: Q9D2Y4

Source: Recombinant protein of mouse MLKL

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:500-2000)

Predicted Molecular Weight: 54 kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shasha Chen. et al. Quercetin alleviates zearalenone-induced apoptosis and necroptosis of porcine renal epithelial cells by inhibiting CaSR/CaMKII signaling pathway. FOOD CHEM TOXICOL. 2023 Nov;:114184Read more>>
VALIDATION IMAGES

Lane 1: Jurkat cell lysates; Lane 2: NCI-H460 cell lysates probed with MLKL Polyclonal Antibody, Unconjugated (bs-55131R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Liver lysates probed with MLKL Polyclonal Antibody, Unconjugated (bs-55131R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: MLKL knockout (KO) HeLa cell lysates probed with MLKL Polyclonal Antibody, Unconjugated (bs-55131R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Rat Testis lysates probed with MLKL Polyclonal Antibody, Unconjugated (bs-55131R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with MLKL polyclonal antibody, unconjugated (bs-55131R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.