bs-55145R [Primary Antibody]
NDUFB4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NDUFB4

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4710

Swiss Prot: O95168

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 14kDa/15kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NDUFB4) polyclonal Antibody, Unconjugated (bs-55145R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NDUFB4) polyclonal Antibody, Unconjugated (bs-55145R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Lane 1: U-87MG cell lysates; Lane 2: Hela cell lysates; Lane 3: 293T cell lysates; Lane 4: Mouse Heart lysates; Lane 5: Mouse Kidney lysates; Lane 6: Mouse Liver lysates probed with NDUFB4 Polyclonal Antibody, Unconjugated (bs-55145R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: NDUFB4 knockout (KO) HeLa cell lysates probed with NDUFB4 Polyclonal Antibody, Unconjugated (bs-55145R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human MOLT4 cell lysates; Lane 5: Human U87MG cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human U-2 OS cell lysates probed with NDUFB4 Polyclonal Antibody, Unconjugated (bs-55145R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.